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rabbit polyclonal anti human pde4a antibody  (Proteintech)


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    Proteintech rabbit polyclonal anti human pde4a antibody
    Fig. 3. PDE4/CD271 expression in healthy and pathologic dermis. (A) <t>PDE4A,</t> PDE4B, PDE4C, PDE4D isoforms and CD271 in dermis from healthy controls and patients with psoriasis, AD, and DLE were detected by IHC. Fast red was used as chromogen. Bar (shown in top left image = 200 μm) (B) The expression intensity of PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in (A) was quantitatively measured using ImageJ software as described in the Materials and Methods. Relative staining intensities are shown, normalized to intensities in healthy dermis for each individual antibody.
    Rabbit Polyclonal Anti Human Pde4a Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human pde4a antibody/product/Proteintech
    Average 92 stars, based on 9 article reviews
    rabbit polyclonal anti human pde4a antibody - by Bioz Stars, 2026-03
    92/100 stars

    Images

    1) Product Images from "Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex."

    Article Title: Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.

    Journal: Cellular signalling

    doi: 10.1016/j.cellsig.2016.01.007

    Fig. 3. PDE4/CD271 expression in healthy and pathologic dermis. (A) PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in dermis from healthy controls and patients with psoriasis, AD, and DLE were detected by IHC. Fast red was used as chromogen. Bar (shown in top left image = 200 μm) (B) The expression intensity of PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in (A) was quantitatively measured using ImageJ software as described in the Materials and Methods. Relative staining intensities are shown, normalized to intensities in healthy dermis for each individual antibody.
    Figure Legend Snippet: Fig. 3. PDE4/CD271 expression in healthy and pathologic dermis. (A) PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in dermis from healthy controls and patients with psoriasis, AD, and DLE were detected by IHC. Fast red was used as chromogen. Bar (shown in top left image = 200 μm) (B) The expression intensity of PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in (A) was quantitatively measured using ImageJ software as described in the Materials and Methods. Relative staining intensities are shown, normalized to intensities in healthy dermis for each individual antibody.

    Techniques Used: Expressing, Software, Staining

    Fig. 4. PDE4/CD271 expression in fibroblasts and myofibroblasts in vitro. (A) α-SMA expression in DF and DM obtained after treatment with diluent (control) or TGF-β1 (1 ng/mL) for 6 days by immunofluorescence in situ. Cell nuclei were counterstained with DAPI (blue); bar = 20 μm. (B) After diluent (control) or TGF-β1 treatment, levels of α-SMA were determined by Western blot analysis. Vinculin was used as a loading control. (C) Levels of PDE4A, PDE4B, PDE4C, and PDE4D mRNA in DF and DM were determined by RT-PCR analysis. Bar graph shows the average densitometry values normalized to β-actin. (D) PDE4 isoform expression in DF and DM was determined by Western blot analysis. β-Actin was used as a loading control. Bar graphs show the average densitometry values normalized to β-actin (fold-expression). (E) DF and DM protein extracts were immunoprecipitated with CD271 antibody and immunoblotted with PDE4A, PDE4B, PDE4C, and PDE4D antibodies and with PAS-only as control.
    Figure Legend Snippet: Fig. 4. PDE4/CD271 expression in fibroblasts and myofibroblasts in vitro. (A) α-SMA expression in DF and DM obtained after treatment with diluent (control) or TGF-β1 (1 ng/mL) for 6 days by immunofluorescence in situ. Cell nuclei were counterstained with DAPI (blue); bar = 20 μm. (B) After diluent (control) or TGF-β1 treatment, levels of α-SMA were determined by Western blot analysis. Vinculin was used as a loading control. (C) Levels of PDE4A, PDE4B, PDE4C, and PDE4D mRNA in DF and DM were determined by RT-PCR analysis. Bar graph shows the average densitometry values normalized to β-actin. (D) PDE4 isoform expression in DF and DM was determined by Western blot analysis. β-Actin was used as a loading control. Bar graphs show the average densitometry values normalized to β-actin (fold-expression). (E) DF and DM protein extracts were immunoprecipitated with CD271 antibody and immunoblotted with PDE4A, PDE4B, PDE4C, and PDE4D antibodies and with PAS-only as control.

    Techniques Used: Expressing, In Vitro, Control, In Situ, Western Blot, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation



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    rabbit polyclonal anti human pde4a antibody  (Proteintech)
    92
    Proteintech rabbit polyclonal anti human pde4a antibody
    Fig. 3. PDE4/CD271 expression in healthy and pathologic dermis. (A) <t>PDE4A,</t> PDE4B, PDE4C, PDE4D isoforms and CD271 in dermis from healthy controls and patients with psoriasis, AD, and DLE were detected by IHC. Fast red was used as chromogen. Bar (shown in top left image = 200 μm) (B) The expression intensity of PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in (A) was quantitatively measured using ImageJ software as described in the Materials and Methods. Relative staining intensities are shown, normalized to intensities in healthy dermis for each individual antibody.
    Rabbit Polyclonal Anti Human Pde4a Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit polyclonal anti human pde4a antibody/product/Proteintech
    Average 92 stars, based on 1 article reviews
    rabbit polyclonal anti human pde4a antibody - by Bioz Stars, 2026-03
    92/100 stars
    		  Buy from Supplier

    Image Search Results


    Fig. 3. PDE4/CD271 expression in healthy and pathologic dermis. (A) PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in dermis from healthy controls and patients with psoriasis, AD, and DLE were detected by IHC. Fast red was used as chromogen. Bar (shown in top left image = 200 μm) (B) The expression intensity of PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in (A) was quantitatively measured using ImageJ software as described in the Materials and Methods. Relative staining intensities are shown, normalized to intensities in healthy dermis for each individual antibody.

    Journal: Cellular signalling

    Article Title: Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.

    doi: 10.1016/j.cellsig.2016.01.007

    Figure Lengend Snippet: Fig. 3. PDE4/CD271 expression in healthy and pathologic dermis. (A) PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in dermis from healthy controls and patients with psoriasis, AD, and DLE were detected by IHC. Fast red was used as chromogen. Bar (shown in top left image = 200 μm) (B) The expression intensity of PDE4A, PDE4B, PDE4C, PDE4D isoforms and CD271 in (A) was quantitatively measured using ImageJ software as described in the Materials and Methods. Relative staining intensities are shown, normalized to intensities in healthy dermis for each individual antibody.

    Article Snippet: The blots were blocked for 2 h in blocking buffer (PBS buffer, pH 7.4 with 0.2% Tween 20 and 5% nonfat milk) and incubated with rabbit polyclonal anti-human PDE4A antibody (1:1000; Proteintech Group, Chicago, IL), rabbit polyclonal anti-human PDE4B antibody (1:300; Santa Cruz Biotechnology), rabbit polyclonal anti-human PDE4C antibody (1:300; Santa Cruz Biotechnology), rabbit polyclonal anti-human PDE4D antibody (1:300; Santa Cruz Biotechnology) or mouse monoclonal -SMA (1:3000; Sigma-Aldrich) or mouse monoclonal anti-β-actin (1:1000; AC C EP TE D M AN U SC R IP T Sigma) overnight at 4oC.

    Techniques: Expressing, Software, Staining

    Fig. 4. PDE4/CD271 expression in fibroblasts and myofibroblasts in vitro. (A) α-SMA expression in DF and DM obtained after treatment with diluent (control) or TGF-β1 (1 ng/mL) for 6 days by immunofluorescence in situ. Cell nuclei were counterstained with DAPI (blue); bar = 20 μm. (B) After diluent (control) or TGF-β1 treatment, levels of α-SMA were determined by Western blot analysis. Vinculin was used as a loading control. (C) Levels of PDE4A, PDE4B, PDE4C, and PDE4D mRNA in DF and DM were determined by RT-PCR analysis. Bar graph shows the average densitometry values normalized to β-actin. (D) PDE4 isoform expression in DF and DM was determined by Western blot analysis. β-Actin was used as a loading control. Bar graphs show the average densitometry values normalized to β-actin (fold-expression). (E) DF and DM protein extracts were immunoprecipitated with CD271 antibody and immunoblotted with PDE4A, PDE4B, PDE4C, and PDE4D antibodies and with PAS-only as control.

    Journal: Cellular signalling

    Article Title: Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.

    doi: 10.1016/j.cellsig.2016.01.007

    Figure Lengend Snippet: Fig. 4. PDE4/CD271 expression in fibroblasts and myofibroblasts in vitro. (A) α-SMA expression in DF and DM obtained after treatment with diluent (control) or TGF-β1 (1 ng/mL) for 6 days by immunofluorescence in situ. Cell nuclei were counterstained with DAPI (blue); bar = 20 μm. (B) After diluent (control) or TGF-β1 treatment, levels of α-SMA were determined by Western blot analysis. Vinculin was used as a loading control. (C) Levels of PDE4A, PDE4B, PDE4C, and PDE4D mRNA in DF and DM were determined by RT-PCR analysis. Bar graph shows the average densitometry values normalized to β-actin. (D) PDE4 isoform expression in DF and DM was determined by Western blot analysis. β-Actin was used as a loading control. Bar graphs show the average densitometry values normalized to β-actin (fold-expression). (E) DF and DM protein extracts were immunoprecipitated with CD271 antibody and immunoblotted with PDE4A, PDE4B, PDE4C, and PDE4D antibodies and with PAS-only as control.

    Article Snippet: The blots were blocked for 2 h in blocking buffer (PBS buffer, pH 7.4 with 0.2% Tween 20 and 5% nonfat milk) and incubated with rabbit polyclonal anti-human PDE4A antibody (1:1000; Proteintech Group, Chicago, IL), rabbit polyclonal anti-human PDE4B antibody (1:300; Santa Cruz Biotechnology), rabbit polyclonal anti-human PDE4C antibody (1:300; Santa Cruz Biotechnology), rabbit polyclonal anti-human PDE4D antibody (1:300; Santa Cruz Biotechnology) or mouse monoclonal -SMA (1:3000; Sigma-Aldrich) or mouse monoclonal anti-β-actin (1:1000; AC C EP TE D M AN U SC R IP T Sigma) overnight at 4oC.

    Techniques: Expressing, In Vitro, Control, In Situ, Western Blot, Reverse Transcription Polymerase Chain Reaction, Immunoprecipitation